Hepatoprotective Effect of Methanolic Leaf Extract of Vernonia Amygdalina against Acetaminophen-Induced Hepatotoxicity in Wistar Albino Rats Article by

Abstract

Abstract This study investigated the hepatoprotective effect of methanolic leaf extract of Vernonia amygdalina against acetaminophen-induced hepatotoxicity in Wistar albino rats. Albino rats (wistar strain) were randomly divided into groups of five rats each, Group I served as control and was administered normal saline only, Group II were induced 75.35 mg/kg of acetaminophen, Group III were given 200mg/kg of methanolic leaf extract of Vernonia amygdalina, while Group IV and V were administered 75.35mg/kg of acetaminophen along with different doses (the former were administered 200 mg/kg and the latter were administered 400 mg/kg ) of methanolic leaf extract of Vernonia amygdalina five minutes after the administration of acetaminophen. Administration of the methanolic leaf extract of Vernonia amygdalina were given orally while intra-peritoneal administration was adopted for acetaminophen induced liver damage rats. All administration was done once daily. There after the rats were sacrificed by cervical dislocation and sera collected was used for various biochemical analyses. Liver enzymes (Alanine aminotransferase, Aspartate aminotransferase and Gammal glutamyl transferase), total proteins, albumin and lipid profiles activities were determined. This study showed that administration of the methanolic extract of Vernonia amydalina reduced the damages to the liver against acetaminophen- induced hepatotoxicity in wistar albino rats. Keywords: Methanolic, Hepatoprotective, Vernonia amydalina, Liver enzymes.