Immunochemical Evaluation of Biomarkers of Carcinogenesis, Angiogenesis, Neuro-Cancer Interactions and Demyelination in Cadmium Chloride-Induced Testicular Toxicity in Rats.

Abstract

Cadmium is a carcinogen. Neurotransmitter-cancer interaction and tissue-innervation impact cancer survival. This study examined repro-protective and neuro-protective potentials of MO11 (isolated from Moringa oleifera leaves) and MS06 (isolated from Musa sapientum suckers) in cadmium chloride (CdCl2)-induced testicular toxicity. Twenty-four adult male rats were randomly divided into 6 groups. Group 1 was control. Groups 2-4 and 6 received intraperitoneal single-dose of CdCl2 (Day 1). Groups 3, 4 and 6 were post-treated with MO11-dose, MO11+MS06-doses and Doxorubicin-dose respectively, while Group 5 received Olive Oil-dose (vehicle) from Days 1-17. Quantitative tissue enzyme-linked-immunosorbent-assays of biomarkers of carcinogenesis and neuro-cancer interaction in testicular homogenates were evaluated. Data were analysed using Mann-Whitney-U test (p≤0.05). Results showed downregulations of MBP, Caspase-3 and sVEGFR, but upregulations of Dopamine, Glutamate and Cytochrome-p450 in Groups 3, 4 and 6, compared with Group 2. Overall, CdCl2-induced testicular toxicity, angiogenesis and neurocancer interaction were ameliorated by post-treatments with MO11 and MS06.