Sustainable Use of Sewage Sludge for Marigold (Tagetes erecta L.) Cultivation: Experimental and Predictive Modeling Studies on Heavy Metal Accumulation

Abstract

The present study aimed to investigate the impact of sewage sludge (SS) amendment on the growth, yield, and biochemical attributes of the marigold (Tagetes erecta L. var. Pusa Basanti Gainda) crop. For this purpose, marigold flowers were cultivated using three different treatments of SS, i.e., 0% (control with no SS), 5%, and 10%. Multiple linear regression (MLR) modeling was performed to develop prediction models for the impact of soil properties on heavy metals uptake by marigold plants. The results showed that the growth, yield, and biochemical attributes of marigold plants significantly (p < 0.05) increased with an increase in SS dose from 0 to 10%. The most feasible SS treatment was found to be 10%, which achieved a maximum flower yield of 318.42 g/plant. On the other hand, the bioaccumulation factor (BAF) values (>1) showed that the marigold plant was capable of uptaking significant contents of six heavy metals in the order of Cd < Cr < Cu < Zn < Mn < Fe. The MLR-based predictive models were capable of precisely predicting the contents of most heavy metal uptake by marigold plants as indicated by the coefficient of determination (R2 > 0.73), model efficiency (ME > 0.49), root mean square error (RMSE < 3.25), and analysis of variance (ANOVA; p < 0.05) results. Overall, this study presented a novel approach to floriculture by sustainable management of SS while reducing public health and environmental impacts.